Centrifugation, pH Measurement
Start with the big picture
Centrifugation separates particles according to their mass and density under relative centrifugal force (RCF), which depends on rotor radius as well as speed. Differential centrifugation progressively pellets heavier particles, while density-gradient methods separate particles by buoyant density. Rotor selection, balancing, inspection, and temperature control contribute to safe, consistent operation. Laboratory pH measurement uses a glass combination electrode to compare sample hydrogen-ion activity with an internal reference. Calibration with suitable buffers, temperature compensation, and careful electrode rinsing and storage help maintain measurement quality. Together, these topics connect core physical principles with practical laboratory workflow and quality control.
What you'll learn
- Explain why RCF, rather than RPM alone, is used to describe centrifugation conditions.
- Distinguish differential centrifugation from density-gradient separation.
- Relate rotor design and basic safety practices to centrifuge applications.
- Describe how a glass combination electrode measures pH.
- Identify calibration, temperature, rinsing, and storage practices that support reliable pH readings.
Continue your study
Work through the complete notes and reinforce the topic with the study tools available in the full lesson.
