Lineweaver-Burk Plot
Start with the big picture
The plot graphs 1/V₀ against 1/[S], producing a line whose y-intercept is 1/Vmax, x-intercept is −1/Km, and slope is Km/Vmax. These features connect the graph to the enzyme’s kinetic parameters. Inhibitors produce characteristic changes in line position and intercepts: competitive inhibition changes the slope and x-intercept while leaving the y-intercept unchanged; non-competitive inhibition changes the slope and y-intercept while the x-intercept remains unchanged; uncompetitive inhibition produces parallel lines; and mixed inhibition changes both intercepts and the slope. Interpretation has limits: extrapolation near intercepts can magnify experimental error, and the method relies on assumptions such as steady-state kinetics and a single substrate. Alternative linear plots are also considered.
What you'll learn
- Identify the reciprocal variables plotted on each axis.
- Relate the slope and intercepts to Km and Vmax.
- Distinguish inhibition patterns by their effects on the line.
- Recognize key assumptions and limitations of the plot.
Continue your study
Work through the complete notes and reinforce the topic with the study tools available in the full lesson.
